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Sampling points and method (grab or composite) should be the same as that
specified in the client's NPDES discharge permit or recommended by the
regulatory agency. The choice of bioassay test should also be outlined in your
permit. It may be helpful to supply us with a copy of the permit to ensure
proper testing.
Samples should be collected in clean, new, glass/plastic bottles or "cubitainers" and must be cooled immediately to 4oC. Samples should
also be cooled during compositing if at all possible. Please fill containers
completely leaving no air space to help reduce the loss of volatile
components. See table on following page for the amount of sample required for
each test. Please be aware that some static-renewal chronic bioassays require
3 separate samples to be sent per test. Clients typically ship these on
Monday, Wednesday and Friday (for Saturday delivery) for the test renewals.
Please consult permit to see if this applies.
All samples should be labelled using waterproof ink and include: sample
ID, date and time sampled, company name, name of sampler, and whether a grab
or composite sample. Chain of Custody forms should be completed, signed by the
sampler and submitted with the samples. Chain of Custody forms and labels will
be provided by the laboratory if needed. We recommend enclosing all paperwork
in a large ziplock freezer bag to ensure dryness.
Samples should be packed in coolers with "blue ice" and transported to the
laboratory as soon as possible. NPDES testing must begin within 36 hours of
sample collection. There is no "holding time" for hazardous waste samples,
however, we recommend testing within two weeks of sample collection. As a
general rule, try to ship the sample within 2 hours of sample collection to
allow adequate time for arrival at the lab.
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SHIPPING INSTRUCTIONS: For local clients: samples may be delivered to our
sample receiving counter weekdays from 8:00 a.m. to 5:00 p.m. Maps are
available for those unfamiliar with the Ventura area. Saturday and Sunday deliveries must be arranged in advance. Please notify the lab of the airbill number and expected arrival time when shipping. For additional information, please contact us at
Aquatic Testing Laboratories. Our phone number is (805) 650-0546; our FAX number is (805) 650-0756.
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Test |
Container |
Volume |
Holding Time |
Hazardous Waste |
Glass Jar |
100 gm (25 gm min. for screen) |
none |
|
Acute - NPDES |
Percent Survival |
Static |
plastic cubitainer |
One gallon for most tests 2 gallons for trout 5 gallons for stickleback |
36 hours |
|
Static-renewal |
plastic cubitainer |
One gallon for most tests 5 gallons for trout 10 gallons for stickleback |
36 hours |
|
LC50 |
Static |
plastic cubitainer |
One gallon for most tests 5 gallons for trout 10 gallons for stickleback |
36 hours |
|
Static-renewal |
plastic cubitainer |
2 gallons for most tests 10 gallons for trout 20 gallons for stickleback |
36 hours |
|
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Chronic - NPDES |
Freshwater |
Fathead minnow |
plastic cubitainer |
Continuous Discharges: 2.5 gallons three times within one week (Mon, Wed, & Fri) Intermittent Discharges: 5 gallons (single sample) |
36 hours |
|
Ceriodaphnia |
plastic cubitainer |
Continuous Discharges: One gallon three times within one week (Mon, Wed, & Fri) Intermittent Discharges: One gallon (single sample) |
36 hours |
|
Selenastrum |
plastic/glass bottle |
one liter |
36 hours |
|
3 Species Screen |
plastic cubitainer |
Continuous Discharges: 2.5 gallons three times within one week (Mon, Wed, & Fri) Intermittent Discharges: 5 gallons (single sample) |
36 hours |
|
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Marine |
Menidia |
plastic cubitainer |
Continuous Discharges: 2.5 gallons three times within one week (Mon, Wed, & Fri) Intermittent Discharges: 7 gallons (single sample) |
36 hours |
|
Topsmelt |
plastic cubitainer |
Continuous Discharges: 2.5 gallons three times within one week (Mon, Wed, & Fri) Intermittent Discharges: 5 gallons (single sample) |
36 hours |
|
Abalone |
plastic/glass bottle |
one liter |
36 hours |
|
Giant Kelp |
plastic/glass bottle |
one liter |
36 hours |
|
3 Species Screen |
plastic cubitainer |
Continuous Discharges: 2.5 gallons three times within one week (Mon, Wed, & Fri) Intermittent Discharges: 7 gallons (single sample) |
36 hours |
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