Aquatic Testing Laboratories

Tests Available
Hazardous Waste
California: Title 22 CCR
Storm Water Runoff
NPDES - Acute
NPDES - Chronic
Contact Us

California: Title 22 CCR
This test is used to help determine whether or not a sample should be classified as a hazardous waste under state of California criteria, (these criteria are codified in Chapter 30 of Title 22 of the California Code of Regulations, Section 66696). In accordance to these regulations, the California Department of Fish and Game has developed a Hazardous Waste Testing procedural guidance document, (entitled: "Static Acute Bioassay Procedures for Hazardous Waste Samples"), that we follow when we conduct this type of testing. This manual calls for a two-phase testing approach. Typically, the screen bioassay is conducted first, and then, if needed, followed by the definitive bioassay. Testing consists of mixing sub-samples with standardized laboratory water to produce specific test concentrations. The screen test uses two sample concentrations of 400 and 750 mg/l, which bracket the 500 mg/l state criterion, and is conducted in duplicate. Ten fish are then added to each test tank and monitored for 96 hours. Water quality parameters, such as dissolved oxygen, pH, and temperature and mortalities are recorded daily. The final fish survival rate is used to determine whether or not the sample passes state criteria for non-hazardous waste, namely an LC50 greater than 500 mg/l (in other words, the concentration necessary to kill half of the exposed fish must be greater than 500 mg/1). If the sample narrowly fails the screening test, (40% or greater mortality in the 750 mg/l concentration), a definitive test is recommended. The definitive test uses five concentrations bracketing the 500 mg/l criteria with the resulting data analyzed by computer to determine the LC50. Fathead minnows are typically used in this test, however, the golden shiner minnow or juvenile rainbow trout may be used, depending on regional preference.

SPECIAL CONDITIONS:There is no specified holding time or temperature for samples under this protocol. We prefer samples to be shipped cooled to 4oC. Approximately 100 grams of sample are needed for testing, with 25 grams minimum for screen. Please notify laboratory in advance for rainbow trout or golden shiner tests.

Hazardous Waste » California: Title 22 CCR
Storm Water Runoff » Percent Survival
NPDES - Acute » EPA Protocol
NPDES - Chronic » Fathead Minnow / Water Flea / Algal / Inland Silverside / Abalone / Giant Kelp / Topsmelt